Anti-LAT (phospho Tyr191) Antibody (A308438)

Western blot analysis of extracts of various cell lines, using Anti-LAT (phospho Tyr191) Antibody (A308438) at 1:1,000 dilution. Jurkat cells were treated by CD-3 (10 µg/ml) at 37°C for 2 minutes after serum-starvation overnight. NIH/3T3 cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.