Anti-Mast Cell Chymase Antibody [ARC0614] (A80816)

Name: Anti-Mast Cell Chymase Antibody [ARC0614]
Brand: Antibodies.com
SKU: A80816
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC0614] antibody to Mast Cell Chymase for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Mast Cell Marker

Specifications

Name

Anti-Mast Cell Chymase Antibody [ARC0614]

Description

Rabbit monoclonal [ARC0614] antibody to Mast Cell Chymase.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 148-247 of human Mast Cell Chymase (CMA1) (CMA1) (P23946).

Sequence

GWGRTGVLKPGSDTLQEVKLRLMDPQACSHFRDFDHNLQLCVGNPRKTKSAFKGDSGGPLLCAGVAQGIVSYGRSDAKPPAVFTRISHYRPWINQILQAN

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0614

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

27 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Alpha-chymase, Chymase, CMA1, CYH, CYM, Mast cell protease I

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-Mast Cell Chymase Antibody [ARC0614] (A80816)

Western blot analysis of extracts of various cell lines, using Anti-Mast Cell Chymase Antibody [ARC0614] (A80816) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-Mast Cell Chymase Antibody [ARC0614] (A80816)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-Mast Cell Chymase Antibody [ARC0614] (A80816) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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