Anti-MGMT Antibody [MGMT/7454] - BSA and Azide free (A316692)

Anti-MGMT Antibody [MGMT/7454] - BSA and Azide free

Mouse monoclonal [MGMT/7454] antibody to MGMT.

Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross links at the O6  alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6 position of guanine in DNA (prior to cross link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant

WB, IHC-P

Jurkat or MCF-7 cells. Human tonsil or stomach (IHC).

WB: 1-2 µg/ml, IHC: 1-2 µg/ml

Human

Purified His-tagged MGMT protein.

Mouse

Monoclonal

MGMT/7454

kappa

Protein A/G chromatography.

1 mg/ml

21.646 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

6-O-methylguanine-DNA methyltransferase, Methylated-DNA--protein-cysteine methyltransferase, O-6-methylguanine-DNA-alkyltransferase