Mouse monoclonal [21-1A6] antibody to MHC Class II Monomorphic.
Specificity
This antibody recognises a monomorphic determinant on the chicken B-L molecule, the chicken class II major histocompatibility complex (MHC).The level of B-L expression is reported to increase during the bursal phase of B cell differentiation.
Applications
IP, Flow Cytometry, IHC-Fr
Dilutions
Flow Cytometry: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Chicken
Immunogen
Chicken bursa cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
21-1A6
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Chicken MHC Class II (monomorphic) antibody, clone 21-1A6, recognizes a monomorphic determinant on the chicken B-L molecule, the chicken class II major histocompatibility complex (MHC).The level of B-L expression is reported to increase during the bursal phase of B cell differentiation (Veromaa et al. 1988).
