Anti-MKK6 (phospho Ser207) Antibody (A94430)

1 Citation

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This product is discontinued

Anti-MKK6 (phospho Ser207) Antibody (A94430) has been discontinued and is no longer available.

View all MEK6 Antibodies.

Specifications

Name

Anti-MKK6 (phospho Ser207) Antibody

Description

Rabbit polyclonal antibody to MKK6 (phospho Ser207).

Specificity

This antibody detects endogenous levels of MKK6 only when phosphorylated at Ser207.

Applications

WB, IHC, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:5000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human MKK6 around the phosphorylation site of Ser207 (amino acids 173-222).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

37kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Dual specificity mitogen-activated protein kinase kinase 6, MAP kinase kinase 6, MAP2K6, MAPK/ERK kinase 6, MAPKK 6, MEK 6, PRKMK6, SAPK kinase 3, SAPKK-3, SAPKK3, SKK3, Stress-activated protein kinase kinase 3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Immunohistochemistry - Anti-MKK6 (phospho Ser207) Antibody (A94430)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-MKK6 (phospho Ser207) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Western Blot - Anti-MKK6 (phospho Ser207) Antibody (A94430)

Western blot analysis of lysates from MDA-MB-435 cells using Anti-MKK6 (phospho Ser207) Antibody. The left hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

Product Citations (1)

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Possible therapeutic target MKK3 in blister formation in pemphigus

This publication cites Anti-MKK6 (phospho Ser207) Antibody (A94430).

Article Abstract

Pemphigus is induced by autoantibody-mediated disruption of keratinocyte adhesion, implicating the p38 mitogen-activated protein kinase (MAPK) pathway in its pathogenesis [1]. Previous research highlights the necessity of mitogen-activated protein kinase kinase 3 (MKK3) and MKK6 for full p38 activation [2]. This study aims to elucidate the roles of MKK3 and MKK6 in pemphigus and evaluate their inhibition as a therapeutic approach.

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