Use 10µl of the suggested working dilution to label 106 cells in 100µl
Immunogen
Human T lymphocytes.
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse IgG2a Negative Control is suitable for use as a matched isotype negative control reagent to assess the level of non-specific binding of mouse IgG2a monoclonal antibodies to rat cells. This antibody has been tested and found to be negative on the following rat cells – peripheral blood leucocytes, thymocytes, splenocytes and macrophages.Mouse IgG2a Negative Control (MCA1210) recognizes the human CD45 antigen and therefore human leucocytes may be used as a positive control for this product. THIS PRODUCT IS NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES.
Flow Cytometry - Mouse IgG2a (FITC) - Isotype Control (A282445)
Figure A. PE conjugated mouse anti rat CD106 and Mouse IgG2a (FITC) - Isotype Control (A282445). Figure B. PE conjugated mouse anti rat CD106 and Anti-CD11b Antibody [ED8] (FITC) (A280336). All experiments performed on red cell lysed rat bone marrow gated on mononuclear cells.
Flow Cytometry - Mouse IgG2a (FITC) - Isotype Control (A282445)
Figure A. Anti-SIRP alpha Antibody [ED9] (PE) (A280340) and Mouse IgG2a (FITC) - Isotype Control (A282445). Figure B. Anti-SIRP alpha Antibody [ED9] (PE) (A280340) and FITC conjugated mouse anti rat CD11c. All experiments performed on rat peritoneal macrophages.
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