Use 10µl of the suggested working dilution to label 106 cells in 100µl
Immunogen
Human T lymphocytes.
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse IgG2a Negative Control is suitable for use as a matched isotype negative control reagent to assess the level of non-specific binding of mouse IgG2a monoclonal antibodies to rat cells. This antibody has been tested and found to be negative on the following rat cells – peripheral blood leucocytes, thymocytes, splenocytes and macrophages.Mouse IgG2a Negative Control (MCA1210) recognizes the human CD45 antigen and therefore human leucocytes may be used as a positive control for this product. THIS PRODUCT IS NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES.
Flow Cytometry - Mouse IgG2a (PE) - Isotype Control (A282636)
Figure A. FITC conjugated mouse anti rat CD3 and Mouse IgG2a (PE) - Isotype Control (A282636). Figure B. FITC conjugated mouse anti rat CD3 and RPE conjugated mouse anti rat CD2. All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Flow Cytometry - Mouse IgG2a (PE) - Isotype Control (A282636)
Figure A. A647 conjugated mouse anti rat CD4 and Mouse IgG2a (PE) - Isotype Control (A282636). Figure B. A647 conjugated mouse anti rat CD4 and Anti-CD44 Antibody [OX-50] (PE) (A281470). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
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