Anti-MRP8 Antibody (A82762)

Name: Anti-MRP8 Antibody
Brand: Antibodies.com
SKU: A82762
Price: 115.0 GBP
Availability: InStock

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$495

Goat polyclonal antibody to MRP8 for ELISA, WB, IHC, IF and Flow Cytometry.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-MRP8 Antibody

Description

Goat polyclonal antibody to MRP8.

Applications

ELISA, WB, IHC, IF, Flow Cytometry

Dilutions

ELISA: 1:32,000, WB: 0.5-1.5µg/ml, IHC: 3.75µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region (near C terminus) of human MRP8.

Sequence

C-KVVEFDRPEVLRK

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

125 kDa

Predicted MW

150kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

60B8Ag, AI323541, B8Ag, BEE11, CAGA, Calgranulin-A, Calprotectin L1L subunit, Calprotectin, included, CFAG, CGLA, Chemotactic cytokine CP-10, CP-10, Cystic fibrosis antigen, L1Ag, Leukocyte L1 complex light chain, MA387, MIF, Migration inhibitory factor-related protein 8, MRP-8, Myeloid-related protein 8, Neutrophil cytosolic 7 kDa protein, NIF, p8, Pro-inflammatory S100 cytokine, Protein S100-A8, S100 calcium binding protein A8, S100 calcium binding protein A8 (calgranulin A), S100 calcium-binding protein A8, S100A8, S100A8/S100A9 complex, included, S10A8_HUMAN, Urinary stone protein band A

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-MRP8 Antibody (A82762)

MRP8 expression in Human Testes lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-MRP8 Antibody (A82762) at 0.5µg/ml and detected by chemiluminescence.

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Immunohistochemistry - Anti-MRP8 Antibody (A82762)

MRP8 expression in Human Breast analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-MRP8 Antibody (A82762) at 3.75µg/ml and revealed with alkaline phosphatase (AP).

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Immunofluorescence - Anti-MRP8 Antibody (A82762)

MRP8 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-MRP8 Antibody (A82762) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Flow Cytometry - Anti-MRP8 Antibody (A82762)

MRP8 expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-MRP8 Antibody (A82762) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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