Western blot analysis of extracts of various cell lines, using Anti-MSH6 Antibody (A14392) at 1:300 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using Anti-MSH6 Antibody (A14392). Green:GFP-RNF168 fusion protein expression for DNA damage marker. DAPI was used to stain the cell nuclei (blue). RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
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