Rabbit polyclonal antibody to MSK1 (phospho Ser212).
Specificity
This antibody detects endogenous levels of MSK1 only when phosphorylated at Ser212.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:40000
Reactivity
Human, Mouse
Immunogen
Synthetic peptide derived from human MSK1 around the phosphorylation site of Ser212 (amino acids 181-230).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
89kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
90 kDa ribosomal protein S6 kinase 5, Nuclear mitogen- and stress-activated protein kinase 1, Ribosomal protein S6 kinase alpha-5, RPS6KA5, RSK-like protein kinase, RSKL, S6K-alpha-5
Western Blot - Anti-MSK1 (phospho Ser212) Antibody (A94040)
Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 5' using Anti-MSK1 (phospho Ser212) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-MSK1 (phospho Ser212) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
![Flow Cytometry - Anti-MSK1 Antibody [PCRP-RPS6KA5-1A8] - BSA and Azide free (A253732) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253732_1.jpg?profile=product_alternative)
![Flow Cytometry - Anti-MSK1 Antibody [PCRP-RPS6KA5-1A8] (A250552) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250552_1.jpg?profile=product_alternative)
![Western Blot - Anti-MSK1 Antibody [ARC61540] (A329649) - Antibodies.com](https://cdn.antibodies.com/image/catalog/329/A329649_1.jpg?profile=product_alternative)
