Rabbit polyclonal antibody to MSK1 (phospho Thr581).
Specificity
This antibody detects endogenous levels of MSK1 only when phosphorylated at Thr581.
Applications
WB, IF, ELISA
Dilutions
WB: 1:500~1:1000, IF: 1:100~1:500, ELISA: 1:40000
Reactivity
Human, Mouse
Immunogen
Synthetic peptide derived from human MSK1 around the phosphorylation site of Thr581 (amino acids 551-600).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
89kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
90 kDa ribosomal protein S6 kinase 5, Nuclear mitogen- and stress-activated protein kinase 1, Ribosomal protein S6 kinase alpha-5, RPS6KA5, RSK-like protein kinase, RSKL, S6K-alpha-5
Immunofluorescence analysis of A549 cells using Anti-MSK1 (phospho Thr581) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-MSK1 (phospho Thr581) Antibody (A94242)
Western blot analysis of lysates from RAW264.7 cells treated with UV 5' using Anti-MSK1 (phospho Thr581) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
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