Rabbit polyclonal antibody to Mu Opioid Receptor (phospho Thr376).
Specificity
This antibody targets the phosphorylated form of human Mu Opioid Receptor at residue Thr376. It detects agonist-induced phosphorylation in Western blot analysis.
Applications
WB
Dilutions
WB: 1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Mu Opioid Receptor around the phosphorylation site of Thr376. Immunogen range is 10-12 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Analysis of agonist-induced phosphorylation of Opioid Receptor Mu 1 (Mu Opioid Receptor) at Threonine376. HEK293 cells stably expressing Mu Opioid Receptor were untreated or treated with 10 µM DAMGO or 0.1 µM PMA for 30 min. Lysates were immunoblotted with anti-Mu Opioid Receptor (phospho Thr376) antibody (A334522) at 1:1000 (upper panel). The blot was stripped and reprobed with phosphorylation-independent anti-Mu Opioid Receptor antibody at 1:1000 to confirm equal loading (lower panel).
Dose-dependent phosphorylation analysis of Opioid Receptor Mu 1 (Mu Opioid Receptor) using phosphosite-specific antibodies. HEK293 cells stably expressing Mu Opioid Receptor were untreated or exposed to 1 nM to 1 µM DAMGO for 30 min. Lysates were immunoblotted with anti-Mu Opioid Receptor (phospho Thr370) (A334521), anti-Mu Opioid Receptor (phospho Ser375) (A334520), anti-Mu Opioid Receptor (phospho Thr376) (A334522), or anti-Mu Opioid Receptor (phospho Thr379) antibodies at 1:1000 (upper panels). The blot was stripped and reprobed with phosphorylation-independent anti-Mu Opioid Receptor antibody at 1:1000 to confirm equal loading (lower panel). Serine375 was the primary phosphorylation site.
Agonist-selective phosphorylation analysis of Opioid Receptor Mu 1 (Mu Opioid Receptor) using phosphosite-specific antibodies. HEK293 cells stably expressing Mu Opioid Receptor were untreated or exposed to 10 µM morphine or 10 µM DAMGO for 30 min. Lysates were immunoblotted with anti-Mu Opioid Receptor (phospho Thr370) (A334521), anti-Mu Opioid Receptor (phospho Ser375) (A334520), anti-Mu Opioid Receptor (phospho Thr376) (A334522), or anti-Mu Opioid Receptor (phospho Thr379) antibodies at 1:1000 (upper panels). The blot was stripped and reprobed with phosphorylation-independent anti-Mu Opioid Receptor antibody at 1:1000 to confirm equal loading (lower panel). DAMGO induced multisite phosphorylation, while morphine primarily induced Serine375 phosphorylation.
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