Rabbit polyclonal antibody to Mu Opioid Receptor (phospho Ser375).
Specificity
This antibody specifically targets the phosphorylated form of human Mu Opioid Receptor at residue Ser375. It detects phosphorylation induced by high- and low-efficacy agonists, but not PKC activation, in Western blot analysis. Ser375 phosphorylation regulates Mu Opioid Receptor desensitization, ß-arrestin recruitment, and internalization. It is suitable for subcellular localization by immunocytochemistry.
Applications
WB, ICC, IHC
Dilutions
IHC: 1:50
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Mu Opioid Receptor around the phosphorylation site of Ser375. Immunogen range is 10-12 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunohistochemical detection of agonist-induced Serine375 phosphorylation of µ-Opioid Receptor (Mu Opioid Receptor) in mouse spinal cord. Mice were injected subcutaneously with saline or 0.3 mg/kg fentanyl citrate for 15 min, then transcardially perfused, fixed, and stained in the presence of phosphatase and protease inhibitors. Confocal images of spinal cord coronal sections were stained with anti-Mu Opioid Receptor (phospho Ser375) antibody (A334558).
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