This antibody recognises human Mucin 1, also known as CD227, Cancer antigen 15-3, Episialin or Krebs von den Lungen-6. CD227 is a 1255 amino acid single pass type I transmembrane glycoprotein. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is a breast cancer associated mucin encoded by the Muc-1 gene. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases. Mouse anti Human CD227 antibody, clone VU-4H5 reacts with the APDTR peptide sequence (VTSAPDTRPAPGSTAPPAHG) of human CD227.
Applications
WB, IHC-P, IHC-Fr, ELISA
Reactivity
Human
Immunogen
60-mer tandem repeat of VTSAPDTRPAPGSTAPPAHG, conjugated to Bovine Serum Albumin.
Sequence
60 x VTSAPDTRPAPGSTAPPAHG
Host
Mouse
Clonality
Monoclonal
Clone ID
VU-4H5
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Human CD227 antibody, clone VU-4H5 recognizes human Mucin 1, also known as CD227, Cancer antigen 15-3, Episialin or Krebs von den Lungen-6. CD227 is a 1255 amino acid single pass type I transmembrane glycoprotein. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is a breast cancer associated mucin encoded by the Muc-1 gene. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases.Mouse anti Human CD227 antibody, clone VU-4H5 reacts with the APDTR peptide sequence (VTSAPDTRPAPGSTAPPAHG) of human CD227.
Synonyms
Breast carcinoma-associated antigen DF3, CA 15-3, Cancer antigen 15-3, Carcinoma-associated mucin, CD227, Episialin, H23AG, MUC-1, Mucin-1, PUM
Sandwich ELISA analysis of CD227 expression using Anti-MUC1 Antibody [C595 (NCRC48)] (A279960) as a capture reagent and biotinylated Anti-MUC1 Antibody [VU-4H5] (A281947) as a detection reagent with purified breast cancer antigen as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Microplate Absorbance Reader. Plasma and serum samples diluted 1:5 are indicated in green and orange respectively
Sandwich ELISA analysis of CD227 expression using Mouse anti Human CD227, clone 6A4 as a capture reagent and biotinylated Anti-MUC1 Antibody [VU-4H5] (A281947) as a detection reagent with purified breast cancer antigen as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on a Microplate Absorbance Reader. Plasma and serum samples diluted 1:5 are indicated in green and orange respectively.
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