Western Blot - Anti-Myc Tag Antibody [AMC0504] (A17318)
Western blot analysis of extracts of normal 293T cells and 293T transfected with Myc-NLK protein, using Anti-Myc Tag Antibody [AMC0504] (A17318) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western Blot - Anti-Myc Tag Antibody [AMC0504] (A17318)
Immunoprecipitation analysis of 300µg extracts of 293T cells using 3µg of Anti-Myc Tag Antibody [AMC0504] (A17318). This Western blot was performed on the immunoprecipitate using Anti-Myc Tag Antibody [AMC0504] (A17318) at a dilution of 1:1000.