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Primary Antibodies
NCK1 Antibodies
Anti-NCK1 Antibody [EM-06] (A86308)
Anti-NCK1 Antibody [EM-06] (A86308)
Overview
Specifications
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$200
Product Datasheet
Mouse monoclonal [EM-06] antibody to NCK1 for WB and ICC.
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100µg
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Shipping Information
Freight/Packing Charges:
$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.
Specifications
Name
Anti-NCK1 Antibody [EM-06]
Description
Mouse monoclonal [EM-06] antibody to NCK1.
Specificity
This antibody recognizes NCK1 (NCK alpha), an ubiquitously expressed cytoplasmic SH2/SH3 adaptor protein important for organization of actin cytoskeleton structures.
Applications
WB
,
ICC
Dilutions
WB: 2 µg/ml; Positive Control: Jurkat cell lysate, IHC: 2 µg/ml; Positive Control: human NCK1-transfected COS-7 cells.
Reactivity
Mouse, Human
Immunogen
Purified recombinant second SH3 domain of human Nck1 fused to GST.
Host
Mouse
Clonality
Monoclonal
Clone ID
EM-06
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Cytoplasmic protein NCK1, NCK, NCK adapter protein 1, Nck-1, SH2/SH3 adapter protein NCK-alpha, SH2/SH3 adapter protein NCK1
Isotype Controls
Mouse IgG1 [PPV-06] (A86755)
Mouse IgG1 [MOPC-21] (A86202)
Mouse IgG1 [MOPC-21] - BSA and Azide Free (A86206)
Suitable Secondaries
Goat Anti-Mouse IgG H&L Antibody (AP) (A301438)
Goat Anti-Mouse IgG H&L Antibody (Biotin) (A301439)
Goat Anti-Mouse IgG H&L Antibody (FITC) (A301669)
Goat Anti-Mouse IgG H&L Antibody (HRP) (A301445)
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Scientific Validation Data
Validation Data
(1)
Enlarge Image
Western Blot - Anti-NCK1 Antibody [EM-06] (A86308)
Western blotting analysis of NCK1 in whole cell lysate of mouse lymph node lymphocytes using Anti-NCK1 Antibody (A86308).
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(3)
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![Western Blot - Anti-NCK1 Antibody [EM-06] (A86307) - Antibodies.com](https://cdn.antibodies.com/image/catalog/86/A86308_564.jpg?profile=product_top)
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![Western Blot - Anti-NCK1 Antibody [EM-06] (A86307) - Antibodies.com](https://cdn.antibodies.com/image/catalog/86/A86308_564.jpg?profile=product_image)
![Western Blot - Anti-Nck Antibody [ARC1441] (A308147) - Antibodies.com](https://cdn.antibodies.com/image/catalog/308/A308147_1.jpg?profile=product_alternative)
