Anti-NK-1R Antibody (A83353)

NK-1R expression in HeLa (A) and Neuro2a (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-NK-1R Antibody (A83353) at 1µg/ml (A) or 0.5µg/ml (B) and detected by chemiluminescence.

NK-1R expression in Neuro-2a cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-NK-1R Antibody (A83353) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Plasma membrane and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.