This antibody specifically recognizes the canonical full-length human NK1R receptor, detecting the non-phosphorylated isoform. It is suitable for Western blot analysis, enables immunoprecipitation from brain tissue lysates, and is optimized for immunohistochemistry in cultured cells and tissue sections.
Applications
WB, ICC, IHC
Dilutions
WB: 1,000, IHC: 1:100, ICC: 1:200
Reactivity
Human
Immunogen
Synthetic peptide corresponding to human NK1R (amino acids. 387-407). Immunogen range is 22-22 amino acids.
Sequence
SSRSDSKTMTESFSFSSNVLS
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot confirmation of Tachykinin Receptor 1 (NK1R) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing NK1R were probed with anti-NK1R antibody (A334551) at 1:1000.
Immunocytochemical analysis of Tachykinin Receptor 1 (NK1R) in HEK293 cells. HEK293 cells stably expressing NK1R were untreated or treated with 1 µM Substance P for 30 min and stained with anti-NK1R antibody (A334551) at 1:200. NK1R receptors were localized to the plasma membrane in untreated cells (0 min) and in perinuclear vesicular clusters after 30 min of Substance P exposure.
Immunohistochemical detection of Tachykinin Receptor 1 (NK1R) in pancreatic carcinoma. Pancreatic carcinoma sections were dewaxed, microwaved in citric acid, and stained with anti-NK1R antibody (A334551) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. NK1R receptors were uniformly detected at the plasma membrane of chief cells.
Localization of Tachykinin Receptor 1 (NK1R) in ovarian carcinoma by immunohistochemistry. Ovarian carcinoma sections were dewaxed, microwaved in citric acid, and incubated with anti-NK1R antibody (A334551) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. NK1R receptors were predominantly observed at the plasma membrane of tumor cells.
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