This antibody recognizes NKG2D, a 42 kDa C-type lectin-like activating receptor expressed by NK cells, gamma/delta T cells, and CD8+ T cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
NKL cell line.
Host
Mouse
Clonality
Monoclonal
Clone ID
1D11
Isotype
IgG1
Light Chains
kappa
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
42 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
CD314, D12S2489E, Killer cell lectin-like receptor subfamily K member 1, KLRK1, NK cell receptor D, NKG2-D type II integral membrane protein, NKG2-D-activating NK receptor
Separation of human CD314 positive CD56 positive NK cells (red-filled) from CD314 negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained with Anti-NKG2D Antibody [1D11] (APC) (A86452), (.
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Proteins predicted to interact with NKG2D
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
![Flow Cytometry - Anti-NKG2D Antibody [1D11] (APC) (A86452)](https://cdn.antibodies.com/image/catalog/86/A86452_1.jpg?profile=product_top)
![Flow Cytometry - Anti-NKG2D Antibody [1D11] (APC) (A86452)](https://cdn.antibodies.com/image/catalog/86/A86452_1.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-NKG2D Antibody [1D11] (APC) (A86452)](https://cdn.antibodies.com/image/catalog/86/A86452_1.jpg?profile=product_image)