Recombinant humanized monoclonal antibody to CD20.
Applications
ELISA, FACS, Functional Assay, In Vivo
Reactivity
Human, Cynomolgus Macaque
Host
Humanized
Clonality
Monoclonal
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A affinity chromatography.
Concentration
Reconstitution dependent.
Molecular Weight
This antibody has a predicted MW of 146.32 kDa.
Purity
> 90% (by SDS-PAGE and SEC-HPLC).
Product Form
Lyophilized
Reconstitution
Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, MS4A1
SDS-PAGE analysis of Obinutuzumab Biosimilar - Anti-CD20 Antibody - Low endotoxin, Azide free (A323615) under reducing (R) conditions confirms that the purity of this antibody is greater than 90%.
SEC-HPLC analysis of Obinutuzumab Biosimilar - Anti-CD20 Antibody - Low endotoxin, Azide free (A323615) confirms that the purity of this antibody is greater than 95%.
Immobilized recombinant human CD20 protein (His tag) at 2 µg/ml is bound by Obinutuzumab Biosimilar - Anti-CD20 Antibody - Low endotoxin, Azide free (A323615). EC50 = 0.0054 µg/ml.
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