Western blot analysis of extracts of various cell lines, using Anti-P4HA1 Antibody (A3999) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Alternative products to Anti-P4HA1 Antibody (A9348)