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Anti-p53 (acetyl K120) Antibody [10E5] (A403)

Identification of p53 protein, whose Lys 120 is acetylated, by Western blotting with 10E5 monoclonal antibody. Samples are crude lysates of HCT116 cells: Left lanes are control. Right lanes are cells treated with siRNA to knockdown the expression of a Tip60 interacting protein UHRF1, which results in increase in acetylation of p53 at Lys120. Total p53 was immuno-precipitated with omnipotent anti-p53 monoclonal antibody (DO-1) from the crude extracts and analyzed by Western blotting with anti-p53 antibody (FL393) (upper panel) or anti-p53 acetyl-K120 monoclonal antibody (10E5) (middle panel). The lower panel shows total p53. Immunoprecipitation of p53 acetylated at K120 by 10E5 monoclonal antibody. Crude cell extracts were prepared from H129 cells (p53 negative cell line) expressing only Myc-p53 (first lane), and both Myc-p53 and His-Tip60. In the upper panel, the whole cell extracts were immuno-blotted with anti-Myc, anti-His-tag or anti-a-tublin antibodies. In the lower panel, the extracts were immuno-precipitated with anti-p53 Ac-K120 antibody (10E5) and the precipitates were immuno-blotted with anti-Myc antibody. Acetylation of p53 at K120 is dependent on Tip60 and promoted by over-expression of His-Tip60 Immunofluorescence staining of p53 acetyl-K120 in nuclei of HeLa cells subjected to DNA damage. HeLa cells were treated with 100 nM Doxorubicin for 24 hr, fixed with 4% paraformaldehyde overnight, permealized with 0.25% Triton X-100 in PBS for 10 min. The antibody was used at 1/1,000 dilution Nucleus (DNA) was stained with DAPI
$555
Product Datasheet

Mouse monoclonal (10E5) antibody to p53 (acetyl K120) for WB, IP, IF, FC and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-p53 (acetyl K120) Antibody [10E5]
Description
Mouse monoclonal (10E5) antibody to p53 (acetyl K120).
Applications
WB, IP, IF, FC, ELISA
Dilutions
WB: 1 µg/ml, FC: 1:100
Reactivity
Human
Immunogen
Synthetic peptide containing acetyl Lys315 of human p53 protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
10E5
Isotype
IgG1
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Antigen NY-CO-13, Cellular tumor antigen p53, Phosphoprotein p53, TP53, Tumor suppressor p53
Isotype Controls
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Identification of p53 protein, whose Lys 120 is acetylated, by Western blotting with 10E5 monoclonal antibody. Samples are crude lysates of HCT116 cells: Left lanes are control. Right lanes are cells treated with siRNA to knockdown the expression of a Tip60 interacting protein UHRF1, which results in increase in acetylation of p53 at Lys120. Total p53 was immuno-precipitated with omnipotent anti-p53 monoclonal antibody (DO-1) from the crude extracts and analyzed by Western blotting with anti-p53 antibody (FL393) (upper panel) or anti-p53 acetyl-K120 monoclonal antibody (10E5) (middle panel). The lower panel shows total p53.Enlarge Image

Anti-p53 (acetyl K120) Antibody [10E5] (A403)

Anti-p53 (acetyl K120) Antibody
Immunoprecipitation of p53 acetylated at K120 by 10E5 monoclonal antibody. Crude cell extracts were prepared from H129 cells (p53 negative cell line) expressing only Myc-p53 (first lane), and both Myc-p53 and His-Tip60. In the upper panel, the whole cell extracts were immuno-blotted with anti-Myc, anti-His-tag or anti-a-tublin antibodies. In the lower panel, the extracts were immuno-precipitated with anti-p53 Ac-K120 antibody (10E5) and the precipitates were immuno-blotted with anti-Myc antibody. Acetylation of p53 at K120 is dependent on Tip60 and promoted by over-expression of His-Tip60Enlarge Image

Immunoprecipitation - Anti-p53 (acetyl K120) Antibody [10E5] (A403)

Anti-p53 (acetyl K120) Antibody
Immunofluorescence staining of p53 acetyl-K120 in nuclei of HeLa cells subjected to DNA damage. HeLa cells were treated with 100 nM Doxorubicin for 24 hr, fixed with 4% paraformaldehyde overnight, permealized with 0.25% Triton X-100 in PBS for 10 min. The antibody was used at 1/1,000 dilution Nucleus (DNA) was stained with DAPIEnlarge Image

Immunofluorescence - Anti-p53 (acetyl K120) Antibody [10E5] (A403)

Anti-p53 (acetyl K120) Antibody
Publishing research using Anti-p53 (acetyl K120) Antibody [10E5] (A403)? Please let us know so that we can list the citation on this page.

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Proteins predicted to interact with p53

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score