Rabbit polyclonal antibody to p53 (acetyl Lys370).
Specificity
This antibody detects endogenous levels of p53 protein only when acetylated at Lys370.
Applications
WB, ELISA
Dilutions
WB: 1:500~1:1000, ELISA: 1:10000
Reactivity
Human
Immunogen
Synthetic Acetyl-peptide derived from human p53 around the Acetylation site of Lys370 (amino acids 331-380).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetyl-peptide. The antibody against non-acetyl-peptide was removed by chromatography using non-acetyl-peptide corresponding to the acetylation site.
Molecular Weight
43kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.