Anti-p53 Antibody [DO-7] (A280266)

Anti-p53 Antibody [DO-7]

Mouse monoclonal [DO-7] antibody to p53.

This antibody recognises the human 53 kDa p53 tumour suppressor protein, also known as Cellular tumor antigen p53 or Antigen NY-CO-13, encoded by the TP53 gene. p53 is a 393 amino acid protein with an N-terminal transactivation domain, followed by a proline-rich region and a DNA binding domain in the central core region. The C-terminal region contains a tetramirization domain and a terminal regulatory domain. p53 is intimately involved in a number of signaling pathways controlling cell division, cycling and apoptosis and is thus a potent cancer suppressor. In normal cells the level of p53 expression is low but can be induced by DNA damage or other stress signals. Activation of p53 leads to growth arrest through its interaction with p21, GADD45 and 14-3-3s, DNA repair and potentially apoptosis through interaction with Bax, Apaf-1, PUMA and NoxA. p53 is critically regulated by Mdm2 which can trigger p53 degradation by a ubiquitin dependent system recognises an epitope at the N-terminal end of p53 between amino acids 20-25, binding to both wild type and mutant forms. Clone DO-7 is not expected to recognize the multiple isoforms lacking the N-teminal region.

WB, IHC-P, IHC-Fr, Flow Cytometry, IP

FC 1: 1:50 - 1:100, IHC-P 2: 1:1,000, WB: 1:1,000, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Human, Bovine

This antibody does not cross react with Mouse or Rat

Recombinant human p53.

Mouse

Monoclonal

DO-7

IgG2b

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti p53 antibody, clone DO-7 recognizes the human 53 kDa p53 tumour suppressor protein, also known as Cellular tumor antigen p53 or Antigen NY-CO-13, encoded by the TP53 gene. p53 is a 393 amino acid protein with an N-terminal transactivation domain, followed by a proline-rich region and a DNA binding domain in the central core region. The C-terminal region contains a tetramirization domain and a terminal regulatory domain (Joerger et al. 2010).p53 is intimately involved in a number of signaling pathways controlling cell division, cycling and apoptosis (Haupt et al. 2003) and is thus a potent cancer suppressor. In normal cells the level of p53 expression is low but can be induced by DNA damage or other stress signals (Takagi et al. 2005). Activation of p53 leads to growth arrest through its interaction with p21, GADD45 and 14-3-3s, DNA repair and potentially apoptosis through interaction with Bax, Apaf-1, PUMA and NoxA (Thakur et al. 2010). p53 is critically regulated by Mdm2 which can trigger p53 degradation by a ubiquitin dependent system (Moll and Petrenko 2003) Mouse anti p53 antibody, clone DO-7 recognizes an epitope at the N-terminal end of p53 between amino acids 20-25, binding to both wild type and mutant forms. Clone DO-7 is not expected to recognize the multiple isoforms lacking the N-teminal region.

Antigen NY-CO-13, Cellular tumor antigen p53, Phosphoprotein p53, TP53, Tumor suppressor p53