Recombinant rabbit monoclonal [p53/3156R] antibody to p53 (CF®640R).
Applications
Functional Studies
Positive Control
MDA-MB-231 or A431 cells and breast or colon carcinoma tissue.
Dilutions
Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/mL, WB: 1-2 µg/mL, IHC-P: 1-2 µg/mL. Directly conjugated primary antibodies may require increased working concentrations compared with indirect detection systems. Antibody concentration and assay conditions should be optimized for each application. A suggested starting concentration is 1-2 µg/mL for most assays.
Reactivity
Human, Mouse, Rat, Monkey, Canine, Hamster
Immunogen
SV40-transformed mouse B4 cells.
Host
Rabbit
Clonality
Monoclonal
Clone ID
p53/3156R
Isotype
IgG
Conjugate
CF®640R
Concentration
0.1 mg/mL
Predicted MW
53 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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