Anti-p53 (phospho S392) Antibody [FP3.2 [FPS392]] (A86708)

Anti-p53 (phospho S392) Antibody [FP3.2 [FPS392]]

Mouse monoclonal [FP3.2 [FPS392]] antibody to p53 (phospho S392).

This antibody reacts with human p53 tumour suppressor protein phosphorylated at CKII site (Ser 392).

WB, IHC-P

IHC-P: Standard ABC technique (DAB+), pretreatment: high temperature antigen retrieval (microwave, pressure cooker) in 10 mM citrate buffer pH 6.0 or 1 mM EDTA-NaOH buffer pH 8.0, recommended dilution: 10 µg/ml, incubation: 1 hour at RT; or overnight at 4°C, Positive Control: breast carcinoma with high level of wild-type p53, WB: 1 µg/ml.

Human

KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P]D, corresponding to amino acids 378-393 of human p53.

RHKKLMFKTEGPDS

Mouse

Monoclonal

FP3.2 [FPS392

Protein A chromatography.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Antigen NY-CO-13, Cellular tumor antigen p53, Phosphoprotein p53, TP53, Tumor suppressor p53