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Anti-p53 (phospho Ser315) Antibody (A402)

Identification of Ser315-phosphorylated p53 proteinby Western blotting. Sample: Crude cell extracts of MCF7 untreated (lane 1) and treated with nocodazole at 100 ng/ml for 48 h (lane2). The lower panel is the whole p53 protein identified by omnipotent anti-p53 antibody (DO-1). Immunohistochemistry of stomach cancer. (Formalin/PFA-fixed paraffin-embedded section) Immunofluorescence staining of p53 phosho-Ser315 in nuclei of HeLa cells subjected DNA damage. 1. HeLa cells were treated with 100 nM Doxorubicin for 24 hr, fixed with 4% paraformaldehyde overnight, permealized with 0.25% Triton X-100 in PBS for 10 min. 2. Incubate cells with 1.5% BSA in PBS for 30 min to block unspecific binding of the antibodies. Incubate the cells with 1/1,000 diluted anti-p53(p-315) antibody in 1% BSA in PBS at 4? overnight. 3. Incubate cells with the secondary antibody, goat anti-mouse IgG conjugated with Alex 488, at 1/1,000 dilution in 1% BSA for 1 hr at room temperature. 4. Nucleus (DNA) was stained with DAPI
$590
Product Datasheet

Mouse monoclonal (#18) antibody to p53 (phospho Ser315) for WB, IHC, IF and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-p53 (phospho Ser315) Antibody
Description
Mouse monoclonal (#18) antibody to p53 (phospho Ser315).
Applications
WB, IHC, IF, ELISA
Dilutions
WB: 1:1,000-1:2,000
Reactivity
Human
Immunogen
Synthetic peptide containing phospho Ser315 of human p53 protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
#18
Isotype
IgG2b
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol (filter-sterilized, without Sodium Azide and carrier free).
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Antigen NY-CO-13, Cellular tumor antigen p53, Phosphoprotein p53, TP53, Tumor suppressor p53
Isotype Controls
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Identification of Ser315-phosphorylated p53 proteinby Western blotting. Sample: Crude cell extracts of MCF7 untreated (lane 1) and treated with nocodazole at 100 ng/ml for 48 h (lane2). The lower panel is the whole p53 protein identified by omnipotent anti-p53 antibody (DO-1).Enlarge Image

Anti-p53 (phospho Ser315) Antibody (A402)

Anti-p53 (phospho Ser315) Antibody
Immunohistochemistry of stomach cancer. (Formalin/PFA-fixed paraffin-embedded section)Enlarge Image

Immunohistochemistry - Anti-p53 (phospho Ser315) Antibody (A402)

Anti-p53 (phospho Ser315) Antibody
Immunofluorescence staining of p53 phosho-Ser315 in nuclei of HeLa cells subjected DNA damage. 1. HeLa cells were treated with 100 nM Doxorubicin for 24 hr, fixed with 4% paraformaldehyde overnight, permealized with 0.25% Triton X-100 in PBS for 10 min. 2. Incubate cells with 1.5% BSA in PBS for 30 min to block unspecific binding of the antibodies. Incubate the cells with 1/1,000 diluted anti-p53(p-315) antibody in 1% BSA in PBS at 4? overnight. 3. Incubate cells with the secondary antibody, goat anti-mouse IgG conjugated with Alex 488, at 1/1,000 dilution in 1% BSA for 1 hr at room temperature. 4. Nucleus (DNA) was stained with DAPIEnlarge Image

Immunofluorescence - Anti-p53 (phospho Ser315) Antibody (A402)

Anti-p53 (phospho Ser315) Antibody
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Proteins predicted to interact with p53

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score