Primary Antibodies
Secondary Antibodies
Proteins & Peptides
ELISA Kits
About Us
Contact Us
Antibodies.com Logo Antibodies.com Logo
Sign In/Register
0
ISO 9001:2015 Certified
Live Customer Support
4.5/5 on Trustpilot
100% Quality Guarantee

Anti-p53 (phospho Ser315) Antibody (A402)

Identification of Ser315-phosphorylated p53 proteinby Western blotting. Sample: Crude cell extracts of MCF7 untreated (lane 1) and treated with nocodazole at 100 ng/ml for 48 h (lane2). The lower panel is the whole p53 protein identified by omnipotent anti-p53 antibody (DO-1). Immunohistochemistry of stomach cancer. (Formalin/PFA-fixed paraffin-embedded section) Immunofluorescence staining of p53 phosho-Ser315 in nuclei of HeLa cells subjected DNA damage. 1. HeLa cells were treated with 100 nM Doxorubicin for 24 hr, fixed with 4% paraformaldehyde overnight, permealized with 0.25% Triton X-100 in PBS for 10 min. 2. Incubate cells with 1.5% BSA in PBS for 30 min to block unspecific binding of the antibodies. Incubate the cells with 1/1,000 diluted anti-p53(p-315) antibody in 1% BSA in PBS at 4? overnight. 3. Incubate cells with the secondary antibody, goat anti-mouse IgG conjugated with Alex 488, at 1/1,000 dilution in 1% BSA for 1 hr at room temperature. 4. Nucleus (DNA) was stained with DAPI
$590
Product Datasheet

Mouse monoclonal (#18) antibody to p53 (phospho Ser315) for WB, IHC, IF and ELISA.

100% Guarantee
Price Match Guarantee
Request a Quotation
Custom or Bulk Request

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-p53 (phospho Ser315) Antibody
Description
Mouse monoclonal (#18) antibody to p53 (phospho Ser315).
Applications
WB, IHC, IF, ELISA
Dilutions
WB: 1:1,000-1:2,000
Reactivity
Human
Immunogen
Synthetic peptide containing phospho Ser315 of human p53 protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
#18
Isotype
IgG2b
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol (filter-sterilized, without Sodium Azide and carrier free).
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Antigen NY-CO-13, Cellular tumor antigen p53, Phosphoprotein p53, TP53, Tumor suppressor p53
Isotype Controls
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Identification of Ser315-phosphorylated p53 proteinby Western blotting. Sample: Crude cell extracts of MCF7 untreated (lane 1) and treated with nocodazole at 100 ng/ml for 48 h (lane2). The lower panel is the whole p53 protein identified by omnipotent anti-p53 antibody (DO-1).Enlarge Image

Anti-p53 (phospho Ser315) Antibody (A402)

Anti-p53 (phospho Ser315) Antibody
Immunohistochemistry of stomach cancer. (Formalin/PFA-fixed paraffin-embedded section)Enlarge Image

Immunohistochemistry - Anti-p53 (phospho Ser315) Antibody (A402)

Anti-p53 (phospho Ser315) Antibody
Immunofluorescence staining of p53 phosho-Ser315 in nuclei of HeLa cells subjected DNA damage. 1. HeLa cells were treated with 100 nM Doxorubicin for 24 hr, fixed with 4% paraformaldehyde overnight, permealized with 0.25% Triton X-100 in PBS for 10 min. 2. Incubate cells with 1.5% BSA in PBS for 30 min to block unspecific binding of the antibodies. Incubate the cells with 1/1,000 diluted anti-p53(p-315) antibody in 1% BSA in PBS at 4? overnight. 3. Incubate cells with the secondary antibody, goat anti-mouse IgG conjugated with Alex 488, at 1/1,000 dilution in 1% BSA for 1 hr at room temperature. 4. Nucleus (DNA) was stained with DAPIEnlarge Image

Immunofluorescence - Anti-p53 (phospho Ser315) Antibody (A402)

Anti-p53 (phospho Ser315) Antibody
Publishing research using Anti-p53 (phospho Ser315) Antibody (A402)? Please let us know so that we can list the citation on this page.

Alternative products to Anti-p53 (phospho Ser315) Antibody (A402)

Western Blot - Anti-p53 Antibody (A308277) - Antibodies.com
(7)
A308277
Anti-p53 Antibody
Rabbit polyclonal antibody to p53 for WB, IHC, ICC/IF and IP.
Western Blot - Anti-p53 (phospho Ser33) Antibody (A90465) - Antibodies.com
(5)
A90465
Anti-p53 (phospho Ser33) Antibody
Rabbit polyclonal antibody to p53 (phospho Ser33) for WB, IHC and IP.
Immunohistochemistry - Anti-p53 Antibody (A16771) - Antibodies.com
(3)
A16771
Anti-p53 Antibody
Rabbit polyclonal antibody to p53 for WB, IHC, ICC/IF and IP.
Western Blot - Anti-p53 (phospho Ser37) Antibody (A306674) - Antibodies.com
(2)
A306674
Anti-p53 (phospho Ser37) Antibody
Rabbit polyclonal antibody to p53 (phospho Ser37) for WB and IHC.
Western Blot - Anti-p53 (phospho Ser15) Antibody (A16438) - Antibodies.com
(2)
A16438
Anti-p53 (phospho Ser15) Antibody
Rabbit polyclonal antibody to p53 (phospho Ser15) for WB and IP.
Western Blot - Anti-p53 (phospho Ser392) Antibody (A91789) - Antibodies.com
(2)
A91789
Anti-p53 (phospho Ser392) Antibody
Rabbit polyclonal antibody to p53 (phospho Ser392) for WB.
Western Blot - Anti-p53 (acetyl Lys370) Antibody (A306200) - Antibodies.com
(2)
A306200
Anti-p53 (acetyl Lys370) Antibody [ARC2368]
Rabbit monoclonal antibody to p53 (acetyl Lys370) for WB.
Western Blot - Anti-p53 Antibody (A307215) - Antibodies.com
(2)
A307215
Anti-p53 Antibody [ARC56085]
Rabbit monoclonal [ARC56085] antibody to p53 for WB and IP.
Western Blot - Anti-p53 (phospho Ser46) Antibody (A10961) - Antibodies.com
(2)
A10961
Anti-p53 (phospho Ser46) Antibody
Rabbit polyclonal antibody to p53 (phospho Ser46) for WB and IP.
Western Blot - Anti-p53 (phospho Ser9) Antibody (A12456) - Antibodies.com
(2)
A12456
Anti-p53 (phospho Ser9) Antibody
Rabbit polyclonal antibody to p53 (phospho Ser9) for WB and IP.
Western Blot - Anti-p53 Antibody (A14956) - Antibodies.com
(2)
A14956
Anti-p53 Antibody
Rabbit polyclonal antibody to p53 for WB and IP.
Western Blot - Anti-p53 (phospho Ser392) Antibody (A308124) - Antibodies.com
A308124
Anti-p53 (phospho Ser392) Antibody [ARC0309]
Rabbit monoclonal [ARC0309] antibody to p53 (phospho Ser392) for WB.
Western Blot - Anti-p53 Antibody (A308277) - Antibodies.com
(7)
A308277
Anti-p53 Antibody
Rabbit polyclonal antibody to p53 for WB, IHC, ICC/IF and IP.
Western Blot - Anti-p53 (phospho Ser33) Antibody (A90465) - Antibodies.com
(5)
A90465
Anti-p53 (phospho Ser33) Antibody
Rabbit polyclonal antibody to p53 (phospho Ser33) for WB, IHC and IP.
Immunohistochemistry - Anti-p53 Antibody (A16771) - Antibodies.com
(3)
A16771
Anti-p53 Antibody
Rabbit polyclonal antibody to p53 for WB, IHC, ICC/IF and IP.
Western Blot - Anti-p53 (phospho Ser37) Antibody (A306674) - Antibodies.com
(2)
A306674
Anti-p53 (phospho Ser37) Antibody
Rabbit polyclonal antibody to p53 (phospho Ser37) for WB and IHC.
Western Blot - Anti-p53 (phospho Ser15) Antibody (A16438) - Antibodies.com
(2)
A16438
Anti-p53 (phospho Ser15) Antibody
Rabbit polyclonal antibody to p53 (phospho Ser15) for WB and IP.
Western Blot - Anti-p53 (phospho Ser392) Antibody (A91789) - Antibodies.com
(2)
A91789
Anti-p53 (phospho Ser392) Antibody
Rabbit polyclonal antibody to p53 (phospho Ser392) for WB.
Western Blot - Anti-p53 (acetyl Lys370) Antibody (A306200) - Antibodies.com
(2)
A306200
Anti-p53 (acetyl Lys370) Antibody [ARC2368]
Rabbit monoclonal antibody to p53 (acetyl Lys370) for WB.
Western Blot - Anti-p53 Antibody (A307215) - Antibodies.com
(2)
A307215
Anti-p53 Antibody [ARC56085]
Rabbit monoclonal [ARC56085] antibody to p53 for WB and IP.
Western Blot - Anti-p53 (phospho Ser46) Antibody (A10961) - Antibodies.com
(2)
A10961
Anti-p53 (phospho Ser46) Antibody
Rabbit polyclonal antibody to p53 (phospho Ser46) for WB and IP.
Western Blot - Anti-p53 (phospho Ser9) Antibody (A12456) - Antibodies.com
(2)
A12456
Anti-p53 (phospho Ser9) Antibody
Rabbit polyclonal antibody to p53 (phospho Ser9) for WB and IP.
Western Blot - Anti-p53 Antibody (A14956) - Antibodies.com
(2)
A14956
Anti-p53 Antibody
Rabbit polyclonal antibody to p53 for WB and IP.
Western Blot - Anti-p53 (phospho Ser392) Antibody (A308124) - Antibodies.com
A308124
Anti-p53 (phospho Ser392) Antibody [ARC0309]
Rabbit monoclonal [ARC0309] antibody to p53 (phospho Ser392) for WB.
See all p53 Antibodies

Proteins predicted to interact with p53

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score