Rabbit polyclonal antibody to p53 (Phospho-Ser315)
Specificity
The antibody detects endogenous level of p53 only when phosphorylated at serine 315.
Applications
WB, IHC, ICC/IF
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 315 (S-S-S(p)-P-Q) derived from Human p53.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from Hela cells using p53 (Phospho-Ser315) Antibody #11100 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (Phospho-Ser315) Antibody #11100 (left) or the same antibody preincubated with blocking peptide (right).
