Anti-p53 (phospho Ser392) Antibody (A91789)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-p53 (phospho Ser392) Antibody (A91789) at 1:1,000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37°C for 20 hours. NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 1s.

Western blot analysis of extracts of various cell lines, using Anti-p53 (phospho Ser392) Antibody (A91789) at 1:1,000 dilution. HeLa cells were treated by Paclitaxel (100 nM/ml) at 37°C for 20 hours. HeLa cells were treated by Hydroxyurea (4 mM) at 37°C for 20 hours. C6 cells were treated by Paclitaxel (100 nM) at 37°C for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 20s.