The antibody detects endogenous level of p53 only when phosphorylated at serine 46.
Applications
WB, IF
Dilutions
Western blotting: 1:500~1:1000 Immunofluorescence: 1:100~1:200
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 46 (M-L-S(p)-P-D) derived from Human p53.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
53kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Western blot analysis of extracts from 293 cells, treated with UV+serum or calf intestinal phosphatase (CIP), using p53 (Phospho-Ser46) Antibody #11099.
Publishing research using Anti-p53 (Phospho-Ser46) Antibody (A50565)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-p53 (Phospho-Ser46) Antibody (A50565)
![Immunohistochemistry - Anti-p53 Antibody [rBP53-12] (A250176) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250176_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-p53 Antibody [TP53/2092R] (A250192) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250192_1.jpg?profile=product_alternative)
![SDS-PAGE - Anti-p53 Antibody [PAb122] - BSA and Azide free (A253363) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253363_1.jpg?profile=product_alternative)
