Western Blot - Anti-p53 (phospho Ser6) Antibody (A308225)
Western blot analysis of extracts of MCF7 cells, using Anti-p53 (phospho Ser6) Antibody (A308225) at 1:1,000 dilution. MCF7 cells were treated by ATP(5 mM) at 30°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 90s.
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