This antibody recognizes Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which, however, migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
Applications
IP, WB, IHC-P
Dilutions
IHC-P: Positive Control: tonsil, colon germinal center, WB: 1-2 µg/ml.
Reactivity
Mouse, Human
Immunogen
Recombinant intracellular fragment corresponding to amino acids 97-432 of human Cbp (PAG).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
46 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
CBP, Csk-binding protein, PAG, Phosphoprotein associated with glycosphingolipid-enriched microdomains 1, Transmembrane adapter protein PAG, Transmembrane phosphoprotein Cbp
Western blotting analysis of human PAG using Anti-PAG1 Antibody (A85825) on lysates of Daudi and Ramos cell line under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of rabbit anti-PAG monoclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. PAG was detected around 65 kDa.
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