The antibody recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
Applications
IP, WB, IHC-P
Dilutions
IHC-P: Positive Control: appendix (germinal center of lymphatic follicle), heat-mediated antigen retrieval in citrate buffer pH 6.1, IP: Positive Control: RAJI human Burkitt lymphoma cell line.
Reactivity
Rat, Bovine, Human, Mouse
Immunogen
C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.
Host
Mouse
Clonality
Monoclonal
Clone ID
PAG-C1
Isotype
IgG2b
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
46 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
CBP, Csk-binding protein, PAG, Phosphoprotein associated with glycosphingolipid-enriched microdomains 1, Transmembrane adapter protein PAG, Transmembrane phosphoprotein Cbp
Immunohistochemistry staining (paraffin sections) of PAG/Cbp in germinal center of lymphatic follicle and in dispersed T cells in appendix tissue by Anti-PAG Antibody (A85668). Positive signal in T cells.
Immunoprecipitation of human PAG/Cbp from the lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with Anti-PAG Antibody (A86214). Note: PAG/Cbp is a 46 kDa adaptor protein, which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Lane 1 & 2: Immunoprecipitation with Anti-PAG Antibody (A85668). Lane 3: Immunoprecipitation with Anti-PAG Antibody (polyclonal antibody).
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