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Anti-PAK1 (phospho Thr212) Antibody (A94422)

Western Blot - Anti-PAK1 (phospho Thr212) Antibody (A0539) - Antibodies.com Immunohistochemistry - Anti-PAK1 (phospho Thr212) Antibody (A0539) - Antibodies.com Western Blot - Anti-PAK1 (phospho Thr212) Antibody (A0539) - Antibodies.com
This product is discontinued

Anti-PAK1 (phospho Thr212) Antibody (A94422) has been discontinued and is no longer available.

View all PAK1 Antibodies.

  • Specifications

Name
Anti-PAK1 (phospho Thr212) Antibody
Description
Rabbit polyclonal antibody to PAK1 (phospho Thr212).
Specificity
This antibody detects endogenous levels of PAK1 only when phosphorylated at Thr212.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:1000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human PAK1 around the phosphorylation site of Thr212 (amino acids 178-227).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
60kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Alpha-PAK, p21-activated kinase 1, p65-PAK, PAK-1, Serine/threonine-protein kinase PAK 1
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-PAK1 (phospho Thr212) Antibody (A0539) - Antibodies.comEnlarge Image

Western Blot - Anti-PAK1 (phospho Thr212) Antibody (A94422)

Western blot analysis of lysates from 293 cells treated with etoposide 25uM 1h using Anti-PAK1 (phospho Thr212) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemistry - Anti-PAK1 (phospho Thr212) Antibody (A0539) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-PAK1 (phospho Thr212) Antibody (A94422)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-PAK1 (phospho Thr212) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-PAK1 (phospho Thr212) Antibody (A0539) - Antibodies.comEnlarge Image

Western Blot - Anti-PAK1 (phospho Thr212) Antibody (A94422)

Western blot analysis of 293 cells using Anti-PAK1 (phospho Thr212) Antibody.

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Proteins predicted to interact with PAK1

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.8% Relevancy Score
99.6% Relevancy Score
99.5% Relevancy Score
99.5% Relevancy Score
99.4% Relevancy Score
99.1% Relevancy Score
96.7% Relevancy Score