Rabbit polyclonal antibody to PAK1 (Phospho-Thr212)
Specificity
The antibody detects endogenous level of PAK1 only when phosphorylated at threonine 212.
Applications
WB, IHC, IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 212 (P-V-T(p)-P-T) derived from Human PAK1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
Alpha-PAK, p21-activated kinase 1, p65-PAK, PAK-1, Serine/threonine-protein kinase PAK 1
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1 (Phospho-Thr212) Antibody #11154 (left) or the same antibody preincubated with blocking peptide (right).
![Western Blot - Anti-PAK1 Antibody [ARC0087] (A307809) - Antibodies.com](https://cdn.antibodies.com/image/catalog/307/A307809_1.jpg?profile=product_alternative)