Rabbit polyclonal antibody to PAK2 (Phospho-Ser141)
Specificity
The antibody detects endogenous levels of PAK2 only when phosphorylated at serine 141.
Applications
WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 141 (Y-L-S(p)-F-T) derived from Human PAK2.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from HepG2 cells treated with Adriamycin (0.5uM, 24hours) and Jurkat cells treated with PMA (125ng/ml, 30mins) using PAK2 (Phospho-Ser141) antibody #12117. The lane on the right is treated with the synthesized peptide.
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