Anti-Parvalbumin Antibody (A85316)

Adult rat cerebellum floating section was stained with Anti-Parvalbumin Antibody, at a dilution of 1:2,500, in green. Parvalbumin is prominently expressed in the dendrites and perikarya of Purkinje cells and the molecular layer interneurons. Blue is a DNA stain.

Immunofluorescent analysis of a rat cerebellum section stained with Anti-Parvalbumin Antibody, at a dilution of 1:2,000, in green, and Anti-GFAP Antibody (A85422 | 1:500, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free floating sections were stained with above antibodies. The Anti-Parvalbumin Antibody labels the perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The Anti-GFAP Antibody stains the processes of Bergmann glia and astrocytes.

Western blot analysis of skeletal muscle lysates and His-tagged recombinant human proteins using Anti-Parvalbumin Antibody, at a dilution of 1:2,000, in green,: [Lane 1] protein standard (red), [Lane 2] mouse muscle, [Lane 3] recombinant parvalbumin, [Lane 4] recombinant calretinin and [Lane 5] recombinant calbindin. Band at 12kDa in muscle lysate is native parvalbumin and 18kDa band in the next lane is His-tagged recombinant parvalbumin. The Anti-Parvalbumin Antibody binds parvalbumin strongly but is not cross-reactive with the closely related calcium binding proteins calretinin or calbindin.

Immunofluorescent analysis of an adult rat cerebellum section stained with Anti-GFAP Antibody [2A5], at a dilution of 1:500, in red, and co-stained with Anti-Parvalbumin Antibody (A85316), at a dilution of 1:2,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and free-floating 45µM sections were stained with above antibodies. Anti-GFAP Antibody [2A5] stains the processes of Bergmann glia and astrocytes. Anti-Parvalbumin Antibody (A85316) labels perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The staining on rodent tissues is specific but not as robust as on human material.