Anti-PDK4 Antibody (A48497)

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This product is discontinued

Anti-PDK4 Antibody (A48497) has been discontinued and is no longer available.

View all PDK4 Antibodies.

Specifications

Name

Anti-PDK4 Antibody

Description

Rabbit polyclonal antibody to PDK4

Specificity

The antibody detects endogenous level of total PDK4 protein.

Applications

E, WB, IHC

Dilutions

ELISA: 1:1000-1:5000 Western blotting: 1:500-1:2000 Immunohistochemistry: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to a region derived from 101-113 amino acids of Human pyruvate dehydrogenase kinase, isozyme 4

Host

Rabbit

Clonality

Polyclonal

Conjugate

Unconjugated

Predicted MW

46kd

Formulation

Supplied at 1.6mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.

Storage

Store at -20˚C / 1 year

Synonyms

PDHK4, Pyruvate dehydrogenase kinase isoform 4, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Anti-PDK4 Antibody (A48497)

Gel: 10% SDS-PAGE Lysate: 60 µg Mouse pancreas tissue lysate Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes

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Anti-PDK4 Antibody (A48497)

The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using 31258 (PDK4 Antibody) at dilution 1/50, on the right is treated with the synthetic peptide.