Anti-PDK4 Antibody (A90016)

Name: Anti-PDK4 Antibody
Brand: Antibodies.com
SKU: A90016
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to PDK4 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-PDK4 Antibody

Description

Rabbit polyclonal antibody to PDK4.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 312-411 of human PDK4 (NP_002603.1).

Sequence

STAPTPVMDNSRNAPLAGFGYGLPISRLYAKYFQGDLNLYSLSGYGTDAIIYLKALSSESIEKLPVFNKSAFKHYQMSSEADDWCIPSREPKNLAKEVAM

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

46 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

PDHK4, Pyruvate dehydrogenase kinase isoform 4, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-PDK4 Antibody (A90016)

Western blot analysis of various lysates, using Anti-PDK4 Antibody (A90016) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunohistochemistry - Anti-PDK4 Antibody (A90016)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-PDK4 Antibody (A90016) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-PDK4 Antibody (A90016)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-PDK4 Antibody (A90016) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-PDK4 Antibody (A90016)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-PDK4 Antibody (A90016) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.