Anti-PHF11 Antibody (A89309)

Name: Anti-PHF11 Antibody
Brand: Antibodies.com
SKU: A89309

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This product is discontinued

Anti-PHF11 Antibody (A89309) has been discontinued and is no longer available.

View all PHF11 Antibodies.

Specifications

Name

Anti-PHF11 Antibody

Description

Rabbit polyclonal antibody to PHF11.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 165-319 of human PHF11 (NP_001035533.1).

Sequence

IAQSAKFSGVKRKRGRKKPLSGNHVQPPETMKCNTFIRQVKEEHGRHTDATVKVPFLKKCKEAGLLNYLLEEILDKVHSIPEKLMDETTSESDYEEIGSALFDCRLFEDTFVNFQAAIEKKIHASQQRWQQLKEEIELLQDLKQTLCSFQENRDL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

38 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BCAP, BRCA1 C-terminus-associated protein, PHD finger protein 11, Renal carcinoma antigen NY-REN-34

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-PHF11 Antibody (A89309)

Western blot analysis of extracts of Mouse brain, using Anti-PHF11 Antibody (A89309) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Immunohistochemistry - Anti-PHF11 Antibody (A89309)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-PHF11 Antibody (A89309) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.