Western blot analysis of extracts from normal (control) and PHKG2 knockout (KO) 293T cells, using Anti-PHKG2 Antibody (A308523) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunofluorescence analysis of C6 cells using Anti-PHKG2 Antibody (A308523) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of L929 cells using Anti-PHKG2 Antibody (A308523) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH-3T3 cells using Anti-PHKG2 Antibody (A308523) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U-2 OS cells using Anti-PHKG2 Antibody (A308523) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
