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Anti-PI 3 Kinase p85 alpha Antibody (A80663)

Western Blot - Anti-PI 3 Kinase p85 alpha Antibody (A80663) - Antibodies.com Immunofluorescence - Anti-PI 3 Kinase p85 alpha Antibody (A80663) - Antibodies.com Immunofluorescence - Anti-PI 3 Kinase p85 alpha Antibody (A80663) - Antibodies.com
This product is discontinued

Anti-PI 3 Kinase p85 alpha Antibody (A80663) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-PI 3 Kinase p85 alpha Antibody
Description
Rabbit polyclonal antibody to PI 3 Kinase p85 alpha.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 125-320 of human PI3 Kinase p85 alpha (NP_852664.1).
Sequence
PDIAPPLLIKLVEAIEKKGLECSTLYRTQSSSNLAELRQLLDCDTPSVDLEMIDVHVLADAFKRYLLDLPNPVIPAAVYSEMISLAPEVQSSEEYIQLLKKLIRSPSIPHQYWLTLQYLLKHFFKLSQTSSKNLLNARVLSEIFSPMLFRFSAASSDNTENLIKVIEILISTEWNERQPAPALPPKPPKPTTVANN
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
85 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-PI 3 Kinase p85 alpha Antibody (A80663) - Antibodies.comEnlarge Image

Western Blot - Anti-PI 3 Kinase p85 alpha Antibody (A80663)

Western blot analysis of various lysates, using Anti-PI 3 Kinase p85 alpha Antibody (A80663) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunofluorescence - Anti-PI 3 Kinase p85 alpha Antibody (A80663) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-PI 3 Kinase p85 alpha Antibody (A80663)

Immunofluorescence analysis of NIH/3T3 cells using Anti-PI 3 Kinase p85 alpha Antibody (A80663) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-PI 3 Kinase p85 alpha Antibody (A80663) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-PI 3 Kinase p85 alpha Antibody (A80663)

Immunofluorescence analysis of PC-12 cells using Anti-PI 3 Kinase p85 alpha Antibody (A80663) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).