Anti-Piccolo Antibody [6H9-B6] (A304747)

Name: Anti-Piccolo Antibody [6H9-B6]
Brand: Antibodies.com
SKU: A304747
Price: 385.0 GBP
Availability: InStock

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$505

Mouse monoclonal [6H9-B6] antibody to Piccolo for WB, ICC/IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-Piccolo Antibody [6H9-B6]

Description

Mouse monoclonal [6H9-B6] antibody to Piccolo.

Applications

WB, ICC/IF, ELISA

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Cross Reactivity

This antibody does not cross-react with Bassoon.

Immunogen

Human full length recombinant protein (GenBank ID: BC001304.1).

Host

Mouse

Clonality

Monoclonal

Clone ID

6H9-B6

Isotype

IgG2b

Light Chains

kappa

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~550 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ACZ, Aczonin, KIAA0559, PCLO, Protein piccolo

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-Piccolo Antibody [6H9-B6] (A304747)

Western blot analysis of rat and mouse brain lysates showing detection of Piccolo protein using Anti-Piccolo Antibody [6H9-B6] (A304747) at 1:1,000. (1) mouse brain lysate; (2) rat brain lysate.

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Immunofluorescence - Anti-Piccolo Antibody [6H9-B6] (A304747)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-Piccolo Antibody [6H9-B6] (A304747), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Cytoplasm, Endoplasmic Reticulum, Nucleus. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Piccolo Antibody. (D) Composite.

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