This antibody recognizes PCLO (Piccolo), a more than 400 kDa multidomain protein expressed mainly in the presynaptic cytomatrix of the neurons.
Applications
Flow Cytometry, WB
Dilutions
Flow Cytometry: 1-5 µg/ml; Intracellular staining, WB: A small splice variant of PCLO can be analyzed by Western blotting, however, the whole protein is too large (over 400 kDa) to be successfully processed by SDS PAAGE.
Reactivity
Human
Immunogen
Human recombinant PCLO protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
PCLO-01
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
400 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western Blot - Anti-Piccolo Antibody [PCLO-01] (A86487)
Western blot of human REH erythroid cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by Anti-PCLO Antibody (A86487) and Goat Anti-Mouse IgG Antibody (H+L) (HRP). The zone evidently corresponds to a small splice variant of the PCLO protein present in this type of cell (see Uniprot database, Genome Res. 14:2121-2127(2004)); the major form (over 500 kDa) expressed in neurons is too large for analysis by Western blotting.
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