Anti-PIM1 Antibody [ARC0175] (A307510)

Name: Anti-PIM1 Antibody [ARC0175]
Brand: Antibodies.com
SKU: A307510
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC0175] antibody to PIM1 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-PIM1 Antibody [ARC0175]

Description

Rabbit monoclonal [ARC0175] antibody to PIM1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PIM1 (P11309).

Sequence

MLLSKINSLAHLRAAPCNDLHATKLAPGKEKEPLESQYQVGPLLGSGGFGSVYSGIRVSDNLPVAIKHVEKDRISDWGELPNGTRVPMEVVLLKKVSSGF

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0175

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

32 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Serine/threonine-protein kinase pim-1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-PIM1 Antibody [ARC0175] (A307510)

Western blot analysis of extracts of 22Rv1 cells, using Anti-PIM1 Antibody [ARC0175] (A307510) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunohistochemistry - Anti-PIM1 Antibody [ARC0175] (A307510)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-PIM1 Antibody [ARC0175] (A307510) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-PIM1 Antibody [ARC0175] (A307510)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-PIM1 Antibody [ARC0175] (A307510) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-PIM1 Antibody [ARC0175] (A307510)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-PIM1 Antibody [ARC0175] (A307510) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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