Western Blot - Anti-PKC alpha (phospho Ser657 + Tyr658) Antibody (A305997)
Western blot analysis of extracts of various cell lines, using Anti-PKC alpha (phospho Ser657 + Tyr658) Antibody (A305997) at 1:1,000 dilution. 293T cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. 293T cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 30s.
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