Western blot analysis of extracts of various cell lines, using Anti-PMS2 Antibody (A88039) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Western blot analysis of extracts from wild type(WT) and PMS2 knockout (KO) HeLa(KO) cells, using Anti-PMS2 Antibody (A88039) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunoprecipitation analysis of 150µg extracts of Jurkat cells using 3µg of Anti-PMS2 Antibody (A88039). This Western blot was performed on the immunoprecipitate using Anti-PMS2 Antibody (A88039) at a dilution of 1:500.
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