Rabbit polyclonal antibody to POLR2A (phospho Ser1619).
Specificity
This antibody detects endogenous levels of POLR2A only when phosphorylated at Ser1619.
Applications
WB, IHC, IF, ELISA
Dilutions
WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:10000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human POLR2A around the phosphorylation site of Ser1619 (amino acids 1585-1634).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing peptide.
Molecular Weight
217kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
3'-5' exoribonuclease, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC:2.7.7.48, POLR2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1
Western Blot - Anti-POLR2A (phospho Ser1619) Antibody (A94903)
Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30' using Anti-POLR2A (phospho Ser1619) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-POLR2A (phospho Ser1619) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunofluorescence analysis of HeLa cells, treated with PMA 125ng/ml 30', using Anti-POLR2A (phospho Ser1619) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
