Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
2C4D, Class II mMOB1, MOB-like protein phocein, Mob1 homolog 3, MOB3, MOB4, MOBKL3, Mps one binder kinase activator-like 3, PHOCN, Preimplantation protein 3
Western blot analysis of extracts of various cell lines, using Anti-PREI3 Antibody (A88964) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-PREI3 Antibody (A88964) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human oophoroma using Anti-PREI3 Antibody (A88964) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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