This antibody recognises rat leumorphin, also known as dynorphin B29. Leumorphin is a 28 amino acid opioid peptide resulting from cleavage of proenkephalin-B. Leumorphin positive cells are found in the paraventricular, supraoptic nucleus and fibres in the lateral hypothalamus of colchicine treated rat brain. Leumorphin has been reported to exert anti-apoptotic effects in rat PC12 pheochromocytoma cells. Rabbit anti leumorphin has been used successfully to detect leumorphin in murine brain using immunohistochemistry on cryostat sections.
Applications
IHC-Fr
Dilutions
IHC-Fr: 1:100 - 1:2,000
Reactivity
Rat, Mouse
Immunogen
Rat Leumorphin, sequence: RSQENPNTYSEDLDV, corresponding to amino acids 14-28.
Sequence
RSQENPNTYSEDLDV
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
Lot Specific
Purity
Serum.
Product Form
Liquid
Formulation
Supplied as serum with <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rabbit anti Leumorphin antibody recognizes rat leumorphin, also known as dynorphin B29. Leumorphin is a 28 amino acid opioid peptide resulting from cleavage of proenkephalin-B. Leumorphin positive cells are found in the paraventricular, supraoptic nucleus and fibres in the lateral hypothalamus of colchicine treated rat brain (Neal & Newman 1989). Leumorphin has been reported to exert anti-apoptotic effects in rat PC12 pheochromocytoma cells (Lee et al. 2005). Rabbit anti leumorphin has been used successfully to detect leumorphin in murine brain using immunohistochemistry on cryostat sections.(Zhang et al.2005).
